• br Methods and materials br

  2018-10-20

  

  Methods and materials Results Discussion Existing methods for hPSC differentiation to endothelial progenitors require the addition of growth factors and/or xenogenic components, limiting their application for large-scale production and therapeutic applications (Bautch, 2011; Wilson et al.,

• br Resource table br Resource details

  2018-10-20

  

  Resource table Resource details An induced pluripotent stem cell (iPSC) line had been generated from human skin fibroblasts of a male, symptomatic 33-year-old spinocerebellar type 2 (SCA2) patient (anonymized as H271) using episomal vectors carrying transcripts for human OCT4, SOX2, KLF4, L-MY

• br Materials and methods br Verification and authentication

  2018-10-20

  

  Materials and methods Verification and authentication Karyotyping was performed at the Center for Applied Human Molecular Genetics, Kennedy Center, Glostrup, Denmark. At least 10 metaphases were analysed per sample with an approximate resolution of 550 to 600 bands per haploid genome. The resu

• br Design of donor plasmids If no

  2018-10-20

  

  Design of donor plasmids If no footprintless gene targeting is required or it is intended to introduce larger transgenes, donor plasmids have to be constructed that contain the homology arms flanking the transgenes or selection cassettes coupled to promoters of choice. Similarly, donor plasmids c

• It has been hypothesized that neurogenesis in DG

  2018-10-20

  

  It has been hypothesized that neurogenesis in DG may play an important role in learning and memory and especially in spatial memory (Deng et al., 2010; Jessberger et al., 2009; Piatti et al., 2013). In the present study, mice with AQ treatment showed an increased percentage of spontaneous alternatio

• We can only speculate on the

  2018-10-20

  

  We can only speculate on the reasons for the contradictions between our study and the Porrello study (Porrello et al., 2011). Importantly, we resected equal amounts of apex tissue, an obvious key parameter that otherwise could have influenced the outcome. Likewise, we used the same time schedule dur

• The relationships of the proximal fetal lung progenitor pool

  2018-10-20

  

  The relationships of the proximal fetal lung progenitor pool characterized in this study to other epithelial cell types remain to be investigated. In the proximal lung of adult animals, either club nos inhibitor expressing SCGB1A1 or basal cells expressing KRT5, ITGA6, and NGFR (and others) acts as

• On the path toward induced pluripotency

  2018-10-20

  

  On the path toward induced pluripotency, investigators rely on gene expression patterns as milestones to gauge appropriate progression, and comparative analysis of publically available gene expression profiles is a common approach. Clearly, our data show that inconsistencies in the relative levels o

• PARKIN PARK an E ubiquitin ligase is the

  2018-10-20

  

  PARKIN (PARK2), an E3 ubiquitin ligase, is the most frequently mutated gene that has casually been linked to autosomal recessive early onset familial Parkinson’s disease (PD) (Abbas et al., 1999; Kitada et al., 1998). Abnormalities of PARK2 have also been described in sporadic PD (Dawson, 2006). The

• promethazine hydrochloride Small molecules have demonstrated

  2018-10-20

  

  Small molecules have demonstrated great potential in modulating cellular fate and functions. A milestone work showed that mouse iPSCs were successfully induced from mouse embryonic fibroblasts (MEF) via six chemical molecules, in the absence of Yamanaka reprogramming factors (Hou et al., 2013). Rece

• ppar agonist Human leukocyte class I HLA I molecules are

  2018-10-20

  

  Human leukocyte class I (HLA-I) molecules are critical for antigen presentation to CD8+ cytotoxic T-lymphocyte (Garrido et al., 1993), and are understood to be widely expressed on the cell surface of all normal human tissues (Daar et al., 1984). However, HLA-I expression has been reported to be abse

• ryanodine Introduction Induced pluripotent stem cells iPSCs

  2018-10-20

  

  Introduction Induced pluripotent stem ryanodine (iPSCs) are stem cells generated by artificial reprogramming of somatic cells with defined factors, such as Oct4, Sox2, Klf4, and c-Myc (Takahashi and Yamanaka, 2006), which are supplied to target cells by exogenous gene expression. iPSC generation c

• Overall our robust transcriptome data in

  2018-10-20

  

  Overall, our robust transcriptome data in buy ITF 2357 undergoing hiPSC reprogramming showed dramatic changes in cell signaling pathways, human-specific AS, and ASE during the progressive cell fate change of fibroblasts to iPSCs (Figure 6E). The data will broaden the knowledge of the reprogramming

• br Acknowledgments We thank members of the Morshead van

  2018-10-20

  

  Acknowledgments We thank members of the Morshead, van der Kooy, and Shoichet groups for helpful discussion. Anand Swaroop kindly provided the Nrl.gfp mouse strain, and Samer Hattar kindly provided TKO mice. We thank Samantha Yammine, Vincent Huynh, and Shannon Sproul for assistance with behaviora

• Previous studies identified TBX as an important driver

  2018-10-20

  

  Previous studies identified TBX3 as an important driver and enhancer for iPSC formation (Han et al., 2010a). In our hands, reprogramming of TBX3-null MEFs occurred with similar efficiencies and kinetics compared to wild-type MEFs. Expanded clones retain the full ground state pluripotency. Interestin

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