On the path toward induced pluripotency

2018-10-20

On the path toward induced pluripotency, investigators rely on gene expression patterns as milestones to gauge appropriate progression, and comparative analysis of publically available gene expression profiles is a common approach. Clearly, our data show that inconsistencies in the relative levels o

PARKIN PARK an E ubiquitin ligase is the

2018-10-20

PARKIN (PARK2), an E3 ubiquitin ligase, is the most frequently mutated gene that has casually been linked to autosomal recessive early onset familial Parkinson’s disease (PD) (Abbas et al., 1999; Kitada et al., 1998). Abnormalities of PARK2 have also been described in sporadic PD (Dawson, 2006). The

promethazine hydrochloride Small molecules have demonstrated

2018-10-20

Small molecules have demonstrated great potential in modulating cellular fate and functions. A milestone work showed that mouse iPSCs were successfully induced from mouse embryonic fibroblasts (MEF) via six chemical molecules, in the absence of Yamanaka reprogramming factors (Hou et al., 2013). Rece

ppar agonist Human leukocyte class I HLA I molecules are

2018-10-20

Human leukocyte class I (HLA-I) molecules are critical for antigen presentation to CD8+ cytotoxic T-lymphocyte (Garrido et al., 1993), and are understood to be widely expressed on the cell surface of all normal human tissues (Daar et al., 1984). However, HLA-I expression has been reported to be abse

ryanodine Introduction Induced pluripotent stem cells iPSCs

2018-10-20

Introduction Induced pluripotent stem ryanodine (iPSCs) are stem cells generated by artificial reprogramming of somatic cells with defined factors, such as Oct4, Sox2, Klf4, and c-Myc (Takahashi and Yamanaka, 2006), which are supplied to target cells by exogenous gene expression. iPSC generation c

Overall our robust transcriptome data in

2018-10-20

Overall, our robust transcriptome data in buy ITF 2357 undergoing hiPSC reprogramming showed dramatic changes in cell signaling pathways, human-specific AS, and ASE during the progressive cell fate change of fibroblasts to iPSCs (Figure 6E). The data will broaden the knowledge of the reprogramming

br Acknowledgments We thank members of the Morshead van

2018-10-20

Acknowledgments We thank members of the Morshead, van der Kooy, and Shoichet groups for helpful discussion. Anand Swaroop kindly provided the Nrl.gfp mouse strain, and Samer Hattar kindly provided TKO mice. We thank Samantha Yammine, Vincent Huynh, and Shannon Sproul for assistance with behaviora

Previous studies identified TBX as an important driver

2018-10-20

Previous studies identified TBX3 as an important driver and enhancer for iPSC formation (Han et al., 2010a). In our hands, reprogramming of TBX3-null MEFs occurred with similar efficiencies and kinetics compared to wild-type MEFs. Expanded clones retain the full ground state pluripotency. Interestin

br Results br Discussion We

2018-10-20

Results Discussion We previously showed that metformin treatment enhances murine adult neurogenesis in vivo without depleting the endogenous NPC pool (Wang et al., 2012). This finding has led to studies to repurpose metformin as a neuroregenerative agent to treat the injured or degenerating bu

Given the robust capacity of these hiNSCs to

2018-10-20

Given the robust capacity of these hiNSCs to maintain neuronal phenotype even in non-neuronal conditions, hiNSCs are highly desirable for multiple tissue engineering applications. When removed from MEF feeders and subsequently grown in generic medium DMEM + 10% FBS, hiNSCs express more than 90% TUJ1

Hello world!

2018-07-29